Coding

Part:BBa_K3429012

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-10-16)
Revision as of 14:42, 22 October 2020 by Phigem (Talk | contribs)


Blue copper oxidase CueO

Profile

Name CueO-Laccase (Spore coat protein A)
Base pairs 1587
Molecular weight 59.23 kDa
Origin Escherichia coli, synthetic
Properties Copper efflux oxidase CueO from Eschericha coli is a copper dependant bacterial laccase possesing five coordinated copper ions relevant for electron transfer. The Cu-ions are coordinated as a T1, three T2/3-cluster Cus and a fifth weakly coordinated copper. CueO differs from most other laccases in its fifth copper binding site.

This part was designed for in vitro characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None