Device

Part:BBa_K3402061

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-27)
Revision as of 13:55, 27 October 2020 by ZZ 0991 (Talk | contribs)


The Cas9 and sgRNA expression cassette

This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), Tsyn7 (BBa_K3402001) and sgPXA1 (BBa_K3402008).

The Cas9 and sgRNA expression cassette.png

Usage and Biology

The use of the strongest promoter Ptef1 to express Cas9 protein and sgRNA could increase the gene-editing efficiency. sgPXA1 will guide the Cas9 to edit the PXA1 site.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5603
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1730
    Illegal BglII site found at 5294
    Illegal XhoI site found at 1051
    Illegal XhoI site found at 1780
    Illegal XhoI site found at 2143
    Illegal XhoI site found at 5095
    Illegal XhoI site found at 6695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5603
    Illegal NgoMIV site found at 2557
    Illegal NgoMIV site found at 3661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1501
    Illegal BsaI site found at 2218


[edit]
Categories
Parameters
None