Composite

Part:BBa_K338062

Designed by: Lucas Hartsough   Group: iGEM10_Caltech   (2010-10-27)


GFP Reporter Device, Heat-Shock or IPTG Induced

Construct assembled to allow the quantitative analysis of the heat shock/LacI promoter/repressor system, BBa_K338002. Produces GFP in response to low-temperature heat shock, more-so in the presence of LacI. GFP production is retarded in LacIq cells (or in the presence of +5mM glucose) about 4-6x. See K338002 for more detailed data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851


[edit]
Categories
//rnap/prokaryote/ecoli/sigma32
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
Parameters
None