Other

Part:BBa_K3365059

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 12:45, 27 October 2020 by Gyaxxxxxx (Talk | contribs)


kanamycin resistant gene with homologous arms

This part is designed to knock out genome rpoZ, which codes for the Omega submit of RNA polymerase. We added 40 base pairs homologous arm at both end of Kanamycin resistant gene in order to replace rpoZ.

Sequencing result firstly.png

Sequencing result secondly.png

Fig:According to the sequencing result, the new sequence, which was rpoZ before, is no longer similar to rpoZ any more, and it is nearly the same sequence as the KanR. This shows that we replace the rpoZ with KanR successfully.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None