Composite

Part:BBa_K3282005

Designed by: Mateusz Piotr Szewczyk, Manjyot Kaur, Julia Thomasson, Savvina Leontakianakou   Group: iGEM19_Lund   (2019-10-11)
Revision as of 16:54, 17 October 2019 by ParselTMatts (Talk | contribs)


pbrD and pbrT under T7 promoter


The part consists of T7 promoter (BBa_I719005), two Anderson RBS (Part:BBa_J61109), separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (Part:BBa_B0012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1444
    Illegal NheI site found at 1642
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1392
    Illegal NgoMIV site found at 1855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1999
    Illegal SapI.rc site found at 2229


[edit]
Categories
Parameters
None