Composite
Part:BBa_K3282005
Designed by: Mateusz Piotr Szewczyk, Manjyot Kaur, Julia Thomasson, Savvina Leontakianakou Group: iGEM19_Lund (2019-10-11)
Revision as of 16:54, 17 October 2019 by ParselTMatts (Talk | contribs)
pbrD and pbrT under T7 promoter
The part consists of T7 promoter (BBa_I719005), two Anderson RBS (Part:BBa_J61109), separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (Part:BBa_B0012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1444
Illegal NheI site found at 1642 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1392
Illegal NgoMIV site found at 1855 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1999
Illegal SapI.rc site found at 2229
[edit]
Categories
Parameters
None |