Coding

Part:BBa_K3282001:Design

Designed by: Manjyot Kaur, Mateusz Piotr Szewczyk   Group: iGEM19_Lund   (2019-10-11)
Revision as of 18:37, 11 October 2019 by ParselTMatts (Talk | contribs) (→‎Source)


C. metallidurans lead transport protein - pbrT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 643
    Illegal NheI site found at 841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 591
    Illegal NgoMIV site found at 1054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1198
    Illegal SapI.rc site found at 1428


Design Notes

It was codon optimized for E. coli Nissle 1917


Source

ACCESSION: NC_006466.1 (115492..117420)

References