Part:BBa_K3282001

C. metallidurans lead transport protein - pbrT

This part contains coding sequence of Cupriavidus metallidurans CH34 lead transport protein (pbrT, Gene ID: 3170370, Uniprot: Q58AJ4) codon-optimized for Escherichia coli Nissle 1917. PbrT is a Pb(II) uptake protein containing a cytochrome domain and a transmembrane metal transport domain (1). It belongs to the ILT (Iron Lead Transporter) family. This protein is a part of the lead (Pb) resistance operon in plasmid pMOL30 (2). Expression of PbrT leads to uptake of lead into the cytoplasm to reduce interaction of free Pb(II) with side chains of membrane and periplasmic proteins, which would cause extensive cellular damage (3).

1. Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., & Lelie, D. V. D. (2008). Lead(II) resistance in Cupriavidus metallidurans CH34: interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek, 96(2), 171–182. doi: 10.1007/s10482-008-9289-0.

2. Hobman, J. L., Julian, D. J., & Brown, N. L. (2012). Cysteine coordination of Pb(II) is involved in the PbrR-dependent activation of the lead-resistance promoter, PbrA, from Cupriavidus metallidurans CH34. BMC Microbiology, 12(1), 109. doi: 10.1186/1471-2180-12-109

3. Borremans, B., Hobman, J. L., Provoost, A., Brown, N. L., & van Der Lelie, D. (2001). Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34. Journal of bacteriology, 183(19), 5651–5658. doi:10.1128/JB.183.19.5651-5658.2001

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 643
  Illegal NheI site found at 841
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 591
  Illegal NgoMIV site found at 1054
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1198
  Illegal SapI.rc site found at 1428