Composite

Part:BBa_K3257122

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-16)
Revision as of 21:26, 21 October 2019 by YY12138 (Talk | contribs)

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mCherry with J23001 Promoter

This part is an expression system for the red fluorescence protein, mCherry.

Prove of well-functional mCherry

Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside E.coli cells.

<span class='h3bbSequence and Features</span>


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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