DNA

Part:BBa_K3257065

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 21:34, 21 October 2019 by YY12138 (Talk | contribs)

lox511

This part is an orthogonal mutant of loxP and it can only interact with the same lox511.

Figure 1. lox511 remains compatible with wildtype loxP, though at a lower excision rate. Wildtype loxP and lox511-mCherry-loxP are analyzed on two different gels, their marker bands are indicated. Wildtype loxP only has an excision band. lox511 has a slight full-length mCherry band slightly longer than 1000 bp, but excision band is still visible and brighter than that of full-length mCherry. This result suggests that lox511 still interacts with wildtype loxP and go through excision, but at a lower efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None