Coding

Part:BBa_K3257033

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-04)
Revision as of 21:19, 21 October 2019 by YY12138 (Talk | contribs)

mCherry

This part is a red fluorescence protein mCherry. We added loxP sites at both ends of it to test if Cre recombinase worked properly.

Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that nonsense mutants lose the ability of fluorescing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None