Composite

Part:BBa_K3239011

Designed by: Xingyu Zhou   Group: iGEM19_ShanghaiFLS_China   (2019-10-16)
Revision as of 07:03, 18 October 2019 by Nicole Guo (Talk | contribs) (→‎Usage and Biology)


pMIT1-PRM1-pPRM1-MIT1

A combination of the pMIT1-PRM1 and the pPRM1-MIT1 constructs.


Usage and Biology

This construct should not only exhibit a combined effect of its two subparts but also the more complex interactions among these two constructs. Figure 1 illustrates the proposed complex regulation of PAOX1.

Figure 1. Illustration of the pGMP-pAPM1-PAOX1-GFP construct (for in trans regulations among homogeneous transcription factors and the homogeneous AOX1, see the PAOX1-GFP construct in part BBa_K3239007










Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2034
    Illegal NheI site found at 3815
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2034
    Illegal BglII site found at 1387
    Illegal BglII site found at 4268
    Illegal BglII site found at 7428
    Illegal BamHI site found at 2917
    Illegal BamHI site found at 3836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2034
    Illegal SpeI site found at 793
    Illegal PstI site found at 2556
    Illegal PstI site found at 6970
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None