Part:BBa_K323153

Scrambled DNA program

This part contains a DNA program - a specifically designed nucleotide sequence containing 6 zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger domains) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Zif268 - PBSII - Jazz - Blues - HivC - Gli1.

We have bound five chimeric enzymes, encoded by Part:BBa_K323135 and Part:BBa_K323132 to the program to show that a correct arrangement of enzymes on the DNA program is important for the progress of the reaction.

[[Image:Slovioscr.png|center|thumb|500px| Figure: Chimeric enzymes for synthesis violacein bound on the scrambled DNA progam.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]