RBS
Spacer+RBS

Part:BBa_K3183026

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-10-16)


Spacer between AgrA and AgrC

This section of DNA provides appropriate separation between the two proteins of the Agr two component signalling system, providing individual purification opportunities.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used in two composite parts in Oxford 2019's ProQuorum project, for detecting C. difficile and lysing it in response using the CD27L endolysin: BBa_K3183102 and BBa_K3183300.

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Categories
Parameters
None