Part:BBa_K3165004

sRBS (250)

sRBS is a strong ribosome binding site present upstream of the transcriptional unit. It has a very strong affinity to bind with the ribosomes allowing for pronounced protein expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

A Ribosome Binding Site (or RBS) is a short DNA sequence present upstream of the transcriptional unit. In prokaryotic systems, it is easily identified by the presence of a Shine-Dalgarno like sequence which pairs with the anti-Shine-Dalgarno like sequence of the 3' end of 16S rRNA. An RBS is crucial for the process of translation as it dictates the binding of the ribosome (the translational machinery) to the mRNA transcript encoding the protein of interest.
The effectiveness (or strength) of the RBS sequence depends on the base-pairing potential of the RBS to the 16S rRNA along with the spacing between the sequence and the start codon. sRBS (250) happens to be a strong ribosome binding site enhancing translational efficiency.

Usage

The sRBS sequence can be used upstream of any gene whose product is desired to be enhanced. This part is used to maximise the translational efficiency of the CcaR protein in the composite gene constructs.

References

(1) Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
PubMed ID: 24608181