Difference between revisions of "Part:BBa K316006"

Revision as of 14:41, 25 October 2010

N-terminus his tagged-GFP-XylE fusion protein

Constructed to be combined with promoter and terminator. The GFP BBa_E0040 has a 5'(5xHis) tagg and is linked to XylE BBa_J33204 monomer subunit (It should be noted that the RBS of J33204 has been removed for this fusion protein). The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.

Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1106
Illegal NgoMIV site found at 1278
Illegal AgeI site found at 1629
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 662

Revision as of 17:03, 24 October 2010 (view source) Ks907 (Talk \| contribs) ← Older edit		Revision as of 14:41, 25 October 2010 (view source) Jec105 (Talk \| contribs) Newer edit →
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−	Constructed to be combined with promoter and terminator. The GFP <bbpart>BBa_E0040</bbpart> has a 5'(5xHis) tagg and is linked to XylE <bbpart>BBa_J33204</bbpart> monomer subunit. The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.	+	Constructed to be combined with promoter and terminator. The GFP <bbpart>BBa_E0040</bbpart> has a 5'(5xHis) tagg and is linked to XylE <bbpart>BBa_J33204</bbpart> monomer subunit (It should be noted that the RBS of J33204 has been removed for this fusion protein). The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.