Part:BBa_K316002

B. subtilis dif: recognition site for sequence-specific endogenous recombinases

In cells with circular chromosomes, recombinatorial repair and homologous recombination can generate multimeric chromosomes 1. ‘’Dif’’ sites are part of a system to ensure that multimeric chromosomes can be separated to monomers, which is required for proper sharing of genetic material between daughter cells. In ‘’B. subtilis’’ the tyrosine family recombinases such as RipX and CodV mediate the separation at 28-bp sequence Bs’’dif’’ 1. The site-specific recombinases are able to recognize two directly repeated ’’dif’’ sites and excise the fragment flanked by the two sites 2.

Figure 1. Removal of a specific gene from a genome integrated construct.

The site-specific recombinases, endogenous to B. subtilis strains are able to recognise Bs’’dif’’ sites and recombine out the strand of DNA directly flanked by the two sites. Recombination leaves a single dif site.

The hypothetical construct in Figure 1, is assumed to have been previously engineered to homologously recombine into the genome of B. subtilis. Integration sequences such as amyE BBa_K143001, BBa_K143002 can be used to achieve this.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>

1. 1 pmid=11208805
2. 2 pmid=16597952

http://2010.igem.org/Team:Imperial_College_London

</biblio>References