Composite

Part:BBa_K3142011:Design

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-10-13)
Revision as of 01:19, 21 October 2019 by Xiaolin (Talk | contribs) (→‎References)

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Complex of 11S globulin from amaranth and hypotensive peptide multimer.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for lactic acid bacteria.

Source

References

Edgar Espinosa-Hernández, et al. The insertion of bioactive peptides at the C-terminal end of an 11S globulin changes the structural stability and improves the antihypertensive activity [J].Electronic Journal of Biotechnology2019, Vol. 37:18–24