Coding

Part:BBa_K3133004

Designed by: Shiyuan Li   Group: iGEM19_Jiangsu_High_School   (2019-10-16)
Revision as of 12:28, 18 October 2019 by Lishiyuan (Talk | contribs)


mTagRFP-mWasabi-LC3

mWasabi-LC3 was used because it was more acid sensitive than EGFP-LC3 in lysosomes and brighter when monitoring autolysosomes in autophagy flux. Also, the exposure time needed by mWasabi-LC3 is shorter than the one of EGFP-LC3, which yielded photos of higher quality. Because LC3 could also be present in non-autophagy cells, mWasabi-LC3∆G is more effective at discriminating them during autophagy flux (Zhou 2012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 622
    Illegal BsaI.rc site found at 760
    Illegal BsaI.rc site found at 1171


[edit]
Categories
Parameters
None