Coding
Part:BBa_K3133004
Designed by: Shiyuan Li Group: iGEM19_Jiangsu_High_School (2019-10-16)
mTagRFP-mWasabi-LC3
mWasabi-LC3 was used because it was more acid sensitive than EGFP-LC3 in lysosomes and brighter when monitoring autolysosomes in autophagy flux. Also, the exposure time needed by mWasabi-LC3 is shorter than the one of EGFP-LC3, which yielded photos of higher quality. Because LC3 could also be present in non-autophagy cells, mWasabi-LC3∆G is more effective at discriminating them during autophagy flux (Zhou 2012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 622
Illegal BsaI.rc site found at 760
Illegal BsaI.rc site found at 1171
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Categories
Parameters
None |