Composite

Part:BBa_K3105681:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)
Revision as of 23:23, 19 October 2019 by JonasG (Talk | contribs) (→‎Design Notes)


Expression construct LiP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this design, overhangs complementary to the pPICZαB vector (Invitrogen easy select Pichia expression kit) were added to No argument specified with partinfo tag. by PCR. This was then assembled into the expression vector generating the circuit in this part

Source

see basic parts

References