Part:BBa_K305011

Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis Bongers, this plasmid was constructed to express proteins present in RCF 10 BioBricks, composed of an RBS followed by a coding sequence, upon subtilin induction.

Usage and Biology

The SURE system is a stringently controlled expression system that displays both high levels of expression and very little promoter 'leakage'. This makes it ideal for the optimization of heterologous protein expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3141
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3147
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3141
  Illegal BglII site found at 3060
  Illegal XhoI site found at 287
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3141
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3141
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3156
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 324
  

References

<biblio>

1. Bongers pmid=16332878

</biblio>