Composite

Part:BBa_K3016200:Design

Designed by: Antti Pennanen, Antti Toivanen   Group: iGEM19_Aalto-Helsinki   (2019-10-16)
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Vibrio natriegens' TorA signal peptide containing YGFP gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 781
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

TorA signal peptide sequence from Vibrio natriegens ATCC 14048 / DSM 759 genomic sequence, NCBI ID NZ_CP016345.1 & NZ_CP016346.1. YGFP gene from Weinstock et al., 2016, supplementary data (see references).

References

Alanen, H. I., Walker, K. L., Suberbie, M. L. V., Matos, C. F., Bönisch, S., Freedman, R. B., ... & Robinson, C. (2015). Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1853(3), 756-763.

Jong, W. S., Vikström, D., Houben, D., de Gier, J. W., & Luirink, J. (2017). Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial cell factories, 16(1), 50.

Sochacki, K. A., Shkel, I. A., Record, M. T., & Weisshaar, J. C. (2011). Protein diffusion in the periplasm of E. coli under osmotic stress. Biophysical journal, 100(1), 22-31.

Weinstock, M. T., Hesek, E. D., Wilson, C. M., & Gibson, D. G. (2016). Vibrio natriegens as a fast-growing host for molecular biology. Nat Methods, 13(10), 849-851. doi:10.1038/nmeth.3970