Coding

Part:BBa_K3013003

Designed by: Gereon Fischer   Group: iGEM19_Aachen   (2019-10-13)
Revision as of 11:31, 19 October 2019 by Gereon (Talk | contribs)


mamC-FbFP BS2 fusion

This fusion protein consists of mamC a glycine linker and a fluorescent protein. MamC is suggested to be located in the magnetosome membrane, wich are produced by magnetotactic bacteria. To report expression of mamC FbFP BS2 is used. This part is an improvement of part BBa_K1094401, because magnetotacic bacteria will produce magnetozomes under anaerobic conditions. In this case there is the need of an anaerobic reporter, as eGFP is dependent to oxygen to develope fluorescence. FbFP BS2 (BBa_K3013002) is flavin mononucleotide-based an independent to oxygen. The glycine linker will help the correct folding of mamC and FbFP BS2.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal NgoMIV site found at 253
    Illegal AgeI site found at 666
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/membrane
//cds/reporter
//function/reporter/fluorescence
Parameters
biology
emission
excitation
protein