RBS

Part:BBa_K2927001

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-28)


Ribosome binding site

Ribosome entry site (RBS) recruits ribosome during the initiation of Cas12a protein translation.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Contributions

Group: iGEM22_Stony_Brook

Authors: Maulik Masaliya

Contribution Summary
This RBS is part of the Protein S gene circuit BBa_K4235030, in the vector BBa_K4235016. We successfully expressed protein S from bacterial expression system as highlighted on our team wiki.


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Categories
Parameters
None