Part:BBa_K2835005

His-tagged fungal laccase with start codon (ATG)

This laccase from Trametes versicolor is originally a ligning degrading enzyme, but it is in fact capable of oxidizing a wide range of aromatic compounds. In our project, it has been used to inactivate the antibiotic sulfamethoxazole, one of the most persistent antibiotics found in the Baltic Sea [1].

This BioBrick is an alternative part to BBa_K2835003, where the only difference between these parts is the present or absent of a start codon (ATG). This Biobrick is a basic part containing a start codon (ATG). This means that this part can be directly ligated (without addition of a start codon through PCR) into any standard iGEM vector and can, after assembly with a promoter, be expressed in a host of choice. The alternative BioBrick BBa_K2835003 is a protein domain not including a start codon, which makes it easy to fuse it to for example a secretion signal or to ligate it into a vector contaning genes which can be fused.

For Usage and Biology as well as characterization, see BBa_K2835003.

References

[1] Helcom (2017). Pharmaceuticals in the aquatic environment of the Baltic Sea region – A status report. Emerging pollutants in water. [online] Paris 07 SP, France: The United Nations Educational, Scientific and Cultural Organization and Helcom.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 547
  Illegal BglII site found at 1243
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]