Difference between revisions of "Part:BBa K2832180"
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== Measurements == | == Measurements == | ||
| − | BBa_K2832180 (apFAB51) was used in the [https://parts.igem.org/Part: | + | BBa_K2832180 (apFAB51) was used in the [https://parts.igem.org/Part:BBa_K3792005 apFAB51 Measurement Device (BBa_K3792005)]. The biological device was inserted in the pSB1K3 plasmid backbone resulting in pFSUIGEM4. Figure 1 is the map of pFSUIGEM4. E. coli NEBExpress was transformed with pFSUIGEM4 resulting in the apFAB51 Test Cell. |
apFAB51 Test Cell was grown in LB Broth medium then fluorescence was measured in a flow cytometer. The results of the measurements are depicted in Figure 2. | apFAB51 Test Cell was grown in LB Broth medium then fluorescence was measured in a flow cytometer. The results of the measurements are depicted in Figure 2. | ||
Revision as of 17:33, 11 October 2023
BIOFAB Modular Promoter apFAB51
BBa_K2832180 is apFAB51 in the BIOFAB Collection . The BIOFAB Collection has constitutive promoters and transcription terminators. The collection is curated by the FSU iGEM teams . BBa_K2832180 (apFAB51) is an engineered constitutive promoter. Information about the design of this promoter is available in the BIOFAB Collectionpage. The FSU iGEM teams are characterizing all the parts in the BIOFAB Collection so they are useful to the iGEM community
Measurements
BBa_K2832180 (apFAB51) was used in the apFAB51 Measurement Device (BBa_K3792005). The biological device was inserted in the pSB1K3 plasmid backbone resulting in pFSUIGEM4. Figure 1 is the map of pFSUIGEM4. E. coli NEBExpress was transformed with pFSUIGEM4 resulting in the apFAB51 Test Cell.
apFAB51 Test Cell was grown in LB Broth medium then fluorescence was measured in a flow cytometer. The results of the measurements are depicted in Figure 2.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]