Difference between revisions of "Part:BBa K274002"

 
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<partinfo>BBa_K274002 short</partinfo>
 
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Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites.  
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Produces a purple pigment (violacein) from L-tryptophane. The operon contains five genes (VioA-E) each with their own ribosome binding sites.  
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[http://2010.igem.org/Team:Slovenia 2010 iGEM team Slovenia] have further characterised the ''vio'' operon ([[Part:BBa_K274002]]) and the ''vio'' operon ABCE ([[Part:BBa_K274004]]). We have analysed the products of both parts by thinlayer liquid cromatography and mass spectrometry (for a detailed description of the procedures see the 2010 iGEM team Slovenia [http://2010.igem.org/Team:Slovenia/METHODS_and_PARTS/protocols/bm#TLC wiki]). For results of our experiments see the page [https://parts.igem.org/Part:BBa_K274002:Experience experience].
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[[Image:SloViolacein-pot.jpg|center|thumb|500px| '''Figure: The violacein biosynthetic pathway.''' Genes for violacein biosynthesis are arranged in an operon consisting of ''vio''A, ''vio''B, ''vio''C, ''vio''D and ''vio''E. VioA generates an IPA imine from L-tryptophan and VioB converts the IPA imine into a dimer. VioE then acts by transforming the dimer into protodexyviolaceinic acid (PVA), which can be spontaneously converted into a green pigment called deoxychromoviridans. VioD and VioC hydroxylate PVA to form violacein.
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Revision as of 21:28, 27 October 2010

Vio operon

Produces a purple pigment (violacein) from L-tryptophane. The operon contains five genes (VioA-E) each with their own ribosome binding sites.

[http://2010.igem.org/Team:Slovenia 2010 iGEM team Slovenia] have further characterised the vio operon (Part:BBa_K274002) and the vio operon ABCE (Part:BBa_K274004). We have analysed the products of both parts by thinlayer liquid cromatography and mass spectrometry (for a detailed description of the procedures see the 2010 iGEM team Slovenia [http://2010.igem.org/Team:Slovenia/METHODS_and_PARTS/protocols/bm#TLC wiki]). For results of our experiments see the page experience.

Figure: The violacein biosynthetic pathway. Genes for violacein biosynthesis are arranged in an operon consisting of vioA, vioB, vioC, vioD and vioE. VioA generates an IPA imine from L-tryptophan and VioB converts the IPA imine into a dimer. VioE then acts by transforming the dimer into protodexyviolaceinic acid (PVA), which can be spontaneously converted into a green pigment called deoxychromoviridans. VioD and VioC hydroxylate PVA to form violacein. .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5602
    Illegal BamHI site found at 4289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6552
    Illegal SapI.rc site found at 6627