Reporter

Part:BBa_K2660001

Designed by: Danielle Biscaro Pedrolli, Paulo Jose Correa Freire   Group: iGEM18_Unesp_Brazil   (2018-10-08)
Revision as of 23:38, 12 October 2018 by Nathanribeiro (Talk | contribs)


T7 promoter driving 6-his tagged GFP with penetratin GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (BBa_K2660000) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750


[edit]
Categories
//chassis/prokaryote
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/production
Parameters
None