Reporter
Part:BBa_K2660001
Designed by: Danielle Biscaro Pedrolli, Paulo Jose Correa Freire Group: iGEM18_Unesp_Brazil (2018-10-08)
Revision as of 23:37, 12 October 2018 by Nathanribeiro (Talk | contribs)
T7 promoter driving 6-his tagged GFP with penetratin
GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (<a href="https://parts.igem.org/Part:BBa_K2660000">BBa_K2660000</a>) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 750
[edit]
Categories
Parameters
//chassis/prokaryote
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/production
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/production
None |