Signalling

Part:BBa_K266000

Designed by: UNAM FCIENCIAS-CINVESTAV IRA   Group: iGEM09_IPN-UNAM-Mexico   (2009-10-13)

PAI+LasR -> LuxI (AI)

This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LasR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
biology
controlPAI
directionForward
negative_regulators
o_h
o_l
positive_regulators1