Regulatory
PDH3

Part:BBa_K2601001

Designed by: Yang Jianzhao   Group: iGEM18_Peking   (2018-10-08)


Promoter-PDH3(Saccharomyces cerevisiae)

We have tested the strength of PDH3 using flow cytometry and compared it with other promoters.

T--Peking--promoter-strength-new.png

Reference

1. Partow, S., Siewers, V., Bjørn, S., Nielsen, J., & Maury, J. (2010). Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast, 27(11), 955-964.‏
2. Yang, C., Hu, S., Zhu, S., Wang, D., Gao, X., & Hong, J. (2015). Characterizing yeast promoters used in Kluyveromyces marxianus. World Journal of Microbiology and Biotechnology, 31(10), 1641-1646.‏

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//promoter
//regulation/positive
Parameters
biologySaccharomyces cerevisiae