Coding

Part:BBa_K2586003

Designed by: Robert Warneke, Janek Meißner   Group: iGEM18_Goettingen   (2018-09-10)
Revision as of 10:18, 11 September 2018 by Dschannek96 (Talk | contribs)


aroE: 3-phosphoshikimate 1-carboxyvinyltransferase

This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in Bacillus subtilis , as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr.
aroE encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. <img src="T--Goettingen--shikimate_pathway_ordinary.png" height="100" width="200" alt="CHIP-Logo" border="2" align="right" hspace="50" vspace="50"> An amplification of aroE allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 90
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
target