Composite

Part:BBa_K2583003

Designed by: LiuJie   Group: iGEM18_Tsinghua-A   (2018-10-03)


GPA1_modify

This part will be used to homologous recombinate with yeast genomic sequence of GPA1 and replace the 5 amino acids of GPA1 with human Gαi1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 585
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2146
    Illegal XhoI site found at 1954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 515
    Illegal BsaI site found at 1482
    Illegal SapI.rc site found at 1329


[edit]
Categories
//chassis/eukaryote/yeast
//function/recombination
Parameters
chassisSaccharomyces cerevisiae
functionmodify yeast GPA1