Coding

Part:BBa_K2560124:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-22)
Revision as of 00:47, 18 October 2018 by Henselt (Talk | contribs)

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Phytobrick version of pTrc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction on the template LVL1 22 + Lux and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Part 1:

Forward oligo: AACGTCTCGCTCGGGAGGTCTAGGGCGGCGGATTTG

Reverse Oligo: AACGTCTCGTGTCACTGGTGAAAAGAAAAACCAC


Part 2:

Forward oligo: AACGTCTCGGACACGGGCAACAGCTG

Reverse Oligo: TTCGTCTCCCTCAAGTAGGTCAGTGCGTCCTGCTG


Source

As source DNA we used LVL1 22 + Lux from the Fritz Lab in Marburg, Germany.