Terminator

Part:BBa_K2560034:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-15)
Revision as of 01:44, 18 October 2018 by Henselt (Talk | contribs) (Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Phytobrick version of BBa_B0010


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by Overlap extension polymerase chain reaction and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGGCTTAACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCC

Reverse Oligo: TTCGTCTCCCTCAAGCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTG


Source

As source we used the part BBa_B0010.