Generator

Part:BBa_K2558201

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)


dCas9 generator with Anderson weak promotor

Part K2558201 is a generator of dCas9, which is a mutant of Cas9 without the DNase activity. dCas9 preserves the ability to bind specific gRNA and then gRNA’s complementary DNA strand. Therefore dCas9/gRNA complex is able to inhibit expression without damaging DNA. As dCas9 is detrimental to the growth of bacteria, we put it after promoter J23114 with B0030 to make sure that dCas9 will be expressed at a constant level with minimal side effect on bacterial growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr
//function/crispr/cas9
Parameters
None