Part:BBa_K2550200
RBS for Toehold Switches
Toehold switches are RNA-based riboregulators functioning as switches that can activate/inhibit reporter expression using a synthetic construct. In the presence of a reverse complementary RNA trigger sequence, reporter expression can be activated through the binding of the trigger sequence to the toehold switch, resulting in the uncoupling of the secondary structure, allowing the RBS to be freed from the "closed" position, allowing for translation of the reporter protein. Toehold switches are advantageous as arbitrary sequences can be utilized to regulate expression of a reporter, serving as unique tools for incorporation as biosensors.
This part encodes an RBS within the stem-loop domain of the toehold switch. In the "closed" position, the RBS is held within the loop, preventing the binding of ribosomes to the RBS, thereby inhibiting translation. In the "open" position, the uncoupling of the RNA switch results in freeing of the RBS. This conformational change allows for binding of ribosomes, thereby allowing translation of the downstream reporter protein to occur.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |