Part:BBa_K2525016
PphlF (PhlF Repressible Promoter)
This part contains a promoter that is repressible by the PhlF repressor, which is a high cooperativity repressor (n = 4.5).
This can be used as a part of a genetic circuit, where the PhlF repressor is present. The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing and ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. Due to its high cooperativity, PhlF can give a much better and steeper curve than most traditional repressors. This is highly desirable.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |