Part:BBa_K2442210

Wild Type RBS from P.fluorescens The wild type RBS taken from the intergenic region mtlR and the mtlE coding region in Pseudomonas Flourescens

Usage and Biology

The RBS was tested within the reporter plasmid assay. The reporter plasmid contained the wild type mtlE promoter upstream of the RBS which was upstream of GFP. In order to test the working of our reporter constructs, these were ligated into E.coli, DH5a cells. We studied the indiction of the wild type reporter plasmid with a number of different sugars, mannitol, sorbitol, sucrose, xylose, ribose, and fructose. The reporter plasmid was tested alone with these sugars as well as being tested alongside the regulatory plasmid.

Figure 1: GFP response to the sugar induction of regulatory and or reporter plasmid

Our results show a GFP response for both the wild type reporter plasmid alone and the wild type reporter plasmid with regulatory plasmid. This gives indication that the wild type RBS works, as there is expression of the GFP protein.

This part was not submitted indivdually, it is part of our composite part, wild type plasmid

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]