Regulatory
CUP1p

Part:BBa_K2407000

Designed by: Mingzhe Han   Group: iGEM17_Tianjin   (2017-10-11)
Revision as of 10:29, 27 October 2017 by MickeyHan (Talk | contribs)


Shortened CUP1 promoter without RFC sites

This is a shortened CUP1 promoter, improved from <partsinfo>BBa_K2165004<partsinfo>. This promoter is designed to be deleted with a few bases on the two ends. We retained 5 ACE1/CUP1 binding sites, 2 TATA boxes, and one initiation element in the promoter. The complex of ACE1 and copper ions will bind the promoter, which causes the activation of CUP1 promoter with TATA boxes' help. In this way, this promoter played its key role with less bases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None