Part:BBa_K2256000

sod2 (Na+/H+antiporter) from Schizosaccharomyces pombe

sod2 encodes a Na+/H+ antiporter enzyme, giving tolerance to high sodium and lithium concentrations. We gave Saccharomyces cerevisiae salt tolerance by transfering this parts inserted into pYES plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 424
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Confirmation that S. cerevisiae expressing sod2 acquired salt tolerance

The growth of S. cerevisiae BY4742 cells expressing sod2 was tested on plates containing sodium.

Fig1. Growth of S. cerevisiae expressing sod2 on plates containing sodium. Serial 100-fold dilutions (100⁻¹～100⁻⁶) of saturated cultures were spotted onto SD-ura medium supplemented with 4.0 % (w/v) NaCl and growth was incubated at 30°C for 3 days.

This figure (fig.1) shows that S. cerevisiae cells that overexpress the sod2 product is more survived on SD-ura medium supplemented with 4.0 % (w/v) NaCl than the counterpart control S. cerevisiae transformed with an empty vector.