Part:BBa_K2225002
AtSultr1;2 for S.cerevisiae (high-affinity sulfate transporter )
AtSultr1;2 is a high affinity sulfate transporter that naturally occurs in Arabidopsis thaliana, but is codon optimized for Saccharomyces cerevisiae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 781
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 849
Illegal NgoMIV site found at 1030 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1227
Illegal BsaI.rc site found at 1503
Design Notes
We optimized the codon-usage for Saccharomyces cerevisiae.
Description & Source
The gene encodes for a high-affinity sulfate transporter that naturally occurs in Arabidopsis thaliana and is expressed at epidermis and cortex of its roots [1]. AtSultr1;2 belongs to a family of sulfate transporters and due to studies with mutants the AtSultr1;2 seems to be the most important one. The mutant having a AtSultr1;1-knockout showed up a phenotype similar to the wildtype while the Sultr1;2-mutant showed differences in the phenotype compared to the wildtype relating to root length, growth and sulphate uptake. This confirms the assumption that AtSultr1;2 plays a major role in the sulfate uptake system [2,3].
References
[1] N. Yoshimoto, E. Inoue, A. Watanabe-Takahashi et al., “Posttranscriptional regulation of high-affinity sulfate transporters in Arabidopsis by sulfur nutrition,” Plant physiology, vol. 145, no. 2, pp. 378–388, 2007.
[2] M. Barberon, P. Berthomieu, M. Clairotte et al., “Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2,” The New phytologist, vol. 180, no. 3, pp. 608–619, 2008.
[3] T. Kataoka, A. Watanabe-Takahashi, N. Hayashi et al., “Vacuolar Sulfate Transporters Are Essential Determinants Controlling Internal Distribution of Sulfate in Arabidopsis,” The Plant Cell, vol. 16, no. 10, pp. 2693–2704, 2004.
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