Part:BBa_K2216002
Malonate-semialdehyde dehydrogenase is responsible for the following reaction: 3-oxopropanoate + CoA + NAD(P) —acetyl-CoA + CO2 + NAD(P)H+H(+) (HOOCCH2CHO+NAD+CoASH—CH3CISCoA+CO2+NADH+H+)
In our project specifically, the enzyme further break down the product of aldehyde dehydrogenase Malonic Semiadelhyde to form metabolite acetyl-CoA, CO2, NADH, and NADPH(Yamada and Jakoby).
The primer for making the parts using PCR is provided:
Forward Primer 5’ 3’ Prefix -- matching primer (coding region) (st.codon) 5’ GTTTCTTC GAATTC GCGGCCGC T TCTAG – ATG GGCAAGATCAGATTAACCACC 3’
Reverse primer 3’ 5’ Suffix (reverse compliment) -- matching primer coding region (reverse compliment) 3’ T ACTAGT A GCGGCCG CTGCAG GAAGAAAC – TTAATATTCACGCGATATGTTGGTT 5’
Citation: E.W. Yamada and W.B. Jakoby. “Aldehyde Oxidation V. Direct Conversion of Malonic Semiadlehyde To Acetyl-Coenzyme A”. The Journal of Biological Chemistry. Vol.235, No.3, March 1960.
//function/degradation
| protein |