Coding

Part:BBa_K2201010

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 13:10, 1 November 2017 by ChrisMWh (Talk | contribs)

Construct comprised of PtNTT2, lacO and Cas9

Construct comprised of PtNTT2, lacO and Cas9

Usage and Biology

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3356
    Illegal NheI site found at 3115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3356
    Illegal BamHI site found at 5394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3356
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None