RBS

Part:BBa_K2138000

Designed by: Austin Nykiel   Group: iGEM16_UMass-Dartmouth   (2016-10-19)


RNA thermometer and RBS (40C)

The RNA thermometer portion of the part is designed to linearize and allow translation of a target protein at forty degrees Celsius. The ribosome binding site was incorporated into the design and selected following the Anderson ribosome binding site format for optimal protein expression and DNA design. The entire part is able to be restricted from the pSB1C3 backbone and ligated with any type of reporter protein to measure temperature regulated expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 118
    Illegal EcoRI site found at 2166
    Illegal XbaI site found at 2181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2166
    Illegal NheI site found at 30
    Illegal NheI site found at 53
    Illegal SpeI site found at 119
    Illegal PstI site found at 133
    Illegal NotI site found at 7
    Illegal NotI site found at 126
    Illegal NotI site found at 2172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2166
    Illegal XhoI site found at 1150
    Illegal XhoI site found at 2042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 119
    Illegal EcoRI site found at 2166
    Illegal XbaI site found at 2181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2166
    Illegal XbaI site found at 16
    Illegal XbaI site found at 2181
    Illegal SpeI site found at 119
    Illegal PstI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None