Coding

Part:BBa_K200028:Design

Designed by: Charles Fracchia, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 15:33, 21 October 2009 by Jec105 (Talk | contribs) (→‎Design Notes)

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Protease resistant PAH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The clone for PAH was obtained from Imagenes (Library : 1643 (Human Liver cDNA), Set : 969 (IRAU MGC Human verified full length cam cDNA), Source: liver))
Site directed mutagenesis was carried out to obtain the desired characteristics

Source

PAH from Homo sapiens.

References