Coding

Part:BBa_K200008:Design

Designed by: Charles Fracchia, Royah Vaezi, Tianyi Wang, Nuri Purswani   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 15:31, 21 October 2009 by Jec105 (Talk | contribs) (→‎Design Notes)

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Phenylalanine hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The clone for PAH was obtained from Imagenes (Library : 1643 (Human Liver cDNA), Set : 969 (IRAU MGC Human verified full length cam cDNA), Source: liver))
Site directed mutagenesis was undertaken in order to remove the XbaI and PstI sites that are present in the Homo sapiens sequence.

Source

Homo Sapiens.

References

http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta