Coding

Part:BBa_K197026:Design

Designed by: John Wang   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:22, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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INP-Repeats


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346		(2296bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5			(EcoRI/BamHI, L)
Product is pBca9495AK-M10048      {<INP repeats>}
---
eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg

References

Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340