Coding

Part:BBa_K197004:Design

Designed by: Patrick Harrigan   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:15, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{VtaA11 AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig113 through O09ig120, assemble by PCA
PCR O09ig113/O09ig120 on PCA reaction        (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig113   {?VtaA11 AtD?}
----
O09ig113     PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG
O09ig114     PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT
O09ig115     PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT
O09ig116     PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA
O09ig117     PCA assembly of {?VtaA11 AtD?} (B09ig113)
CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA
O09ig118     PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA
O09ig119     PCA assembly of {?VtaA11 AtD?} (B09ig113)
ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG
O09ig120     PCA assembly of {?VtaA11 AtD?} (B09ig113)
CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC

References