Coding

Part:BBa_K197001:Design

Designed by: Patrick Harrigan   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:14, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{Azo1653 AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig1 through O09ig26, assemble by PCA
PCR O09ig1/O09ig26 on PCA reaction        (860, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig1   {<azo1653 AtD>}
----
O09ig1     PCA assembly of {<azo1653 AtD>} (B09ig1)
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig2     PCA assembly of {<azo1653 AtD>} (B09ig1)
TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG
O09ig3     PCA assembly of {?azo1653 AtD>} (B09ig1)
CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG
O09ig4     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG
O09ig5     PCA assembly of {?azo1653 AtD?} (B09ig1)
TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT
O09ig6     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA
O09ig7     PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT
O09ig8     PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG
O09ig9     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC
O09ig10     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA
O09ig11     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG
O09ig12     PCA assembly of {?azo1653 AtD?} (B09ig1)
GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC
O09ig13     PCA assembly of {?azo1653 AtD?} (B09ig1)
CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC
O09ig14     PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA
O09ig15     PCA assembly of {?azo1653 AtD?} (B09ig1)
GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG
O09ig16     PCA assembly of {?azo1653 AtD?} (B09ig1)
GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC
O09ig17     PCA assembly of {?azo1653 AtD?} (B09ig1)
CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG
O09ig18     PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC
O09ig19     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG
O09ig20     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG
O09ig21     PCA assembly of {?azo1653 AtD?} (B09ig1)
ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC
O09ig22     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG
O09ig23     PCA assembly of {?azo1653 AtD?} (B09ig1)
TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA
O09ig24     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC
O09ig25     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT
O09ig26     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA

References