RBS

Part:BBa_K1968002

Designed by: Paolo Marangio   Group: iGEM16_Edinburgh_OG   (2016-10-09)


Synechocystis RBS* Phytobrick

RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034.

(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None